Research article

Resolution of complex fluorescence spectra of lipids and nicotinic acetylcholine receptor by multivariate analysis reveals protein-mediated effects on the receptor's immediate lipid microenvironment

Jorge J Wenz and Francisco J Barrantes*

UNESCO Chair of Biophysics and Molecular Neurobiology and Instituto de Investigaciones Bioquímicas de Bahía Blanca, B8000FWB Bahía Blanca, Argentina

For all author emails, please log on.

PMC Biophysics 2008, 1:6 doi:10.1186/1757-5036-1-6

Published: 18 December 2008

Additional File 1:

Reference spectra of AChR, PyPC and NBD-Chol. Fig. 1 and experimental details.

Format: PDF Size: 22KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 2:

REMSEP and explained y-variance. Fig. 2 and extra information about these parameters.

Format: PDF Size: 15KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 3:

FRET controls. Fluorescence emission of AChR-only and PyPC-only samples as a function of temperature. Fig. 3 and experimental details.

Format: PDF Size: 12KB Download file

This file can be viewed with: Adobe Acrobat Reader